Vitamin B12 and Folate Blood Test

Sample Type:
Sample Type
Age: 18+
Collection Method: Visit a Labcorp Location
HSA/FSA Accepted
Short Description

Worried about anemia? Testing these two vitamins levels can help.


Vitamins B12 and folate support many different cellular functions throughout the body. B12 supports nerve function, and folate plays a part in converting carbohydrates into energy as well as producing DNA; both B complex vitamins are also essential for making red blood cells.
Folate is particularly important during pregnancy and plays a role in fetal development. A folate deficiency during pregnancy can increase the risk of neurologic defects to the growing fetus.1
Most individuals get enough folate from foods, especially in the U.S., where all grains and cereal products are fortified with folic acid. However, some people don’t consume enough vitamin B12 to meet their needs, while others can’t absorb enough, no matter how much they take in.2
This test measures both your B12 and folate levels to detect any deficiencies and to diagnose the cause of anemia, if present.




If you’re taking a supplement containing biotin (also called vitamin B7 or B8, vitamin H, or coenzyme R), commonly found in products promoting nail, skin, and hair health, it is recommended that you wait at least 72 hours from your last dose before sample collection.

What's Included

Vitamin B12

Measures the amount of B-12, a water-soluble vitamin that plays a vital role in the health of nerves and red blood cells.

Folate (Folic Acid)

The B vitamin that plays a vital role in making DNA, the genetic material in cells. Folic acid is a synthesized version of folate found in processed foods and supplements. A folate deficiency can lead to health complications, especially in pregnant women.

Why Consider This Test

Early Detection Matters
Early Detection Matters

Early detection and treatment of B12 deficiency is important; if left untreated it can cause severe neurologic problems and blood disorders.2


Pregnant women and women who are breastfeeding have increased demand for folate. Failure to meet this increased demand can result in a deficiency.3,4

At Greater Risk
At Greater Risk

People at risk for B12 and Folate deficiency also include:3,4,5,6,7

Individuals age 65 and older.

Those with conditions that may affect the absorption of B12 in the body such as atrophic gastritis, which is a thinning of the lining of the stomach; pernicious anemia; and conditions such as celiac disease or Crohn’s disease.

Individuals who have had surgery where part of the bowel that absorbs B12 was removed.

People on the drug metformin for diabetes, or chloramphenicol, or those taking long-term antacid drugs, such as proton pump inhibitors or H2 blockers for heartburn, which can affect B12 absorption.

Those following a strict vegetarian or vegan diet.

Chronic alcohol users or those who take certain drugs, such as phenytoin, methotrexate, and sulfa antibiotics, which can affect folate absorption.


  1. Black, Maureen M. “Effects of Vitamin B12 and Folate Deficiency on Brain Development in Children.” Food and Nutrition Bulletin, U.S. National Library of Medicine, June 2008, www.ncbi.nlm.nih.gov/pmc/articles/PMC3137939/.
  2. Skerrett, Patrick J. “Vitamin B12 Deficiency Can Be Sneaky, Harmful.” Harvard Health Blog, 11 Feb. 2019, www.health.harvard.edu/blog/vitamin-b12-deficiency-can-be-sneaky-harmful-201301105780.
  3. “Vitamin B12.” NIH Office of Dietary Supplements, U.S. Department of Health and Human Services, https://ods.od.nih.gov/factsheets/VitaminB12-HealthProfessional
  4. “Folate.” NIH Office of Dietary Supplements, U.S. Department of Health and Human Services, https://ods.od.nih.gov/factsheets/Folate-HealthProfessional/
  5. “Vitamin Deficiency Anemia.” Mayo Clinic, Mayo Foundation for Medical Education and Research, 9 Nov. 2016, www.mayoclinic.org/diseases-conditions/vitamin-deficiency-anemia/symptoms-causes/syc-20355025.

Packages With This Test

Vitamin Deficiency Blood Test:

Are you getting enough of these essential vitamins? Let’s find out.

How To Get This Test

Choose Your Tests
1. Choose Your Tests

Shop for tests and pay online. An independent physician will review and approve your test requests; no doctor’s visit is required.



Provide Your Sample
2. Provide Your Sample

Take the requisition number we emailed you, along with a photo ID, to a Labcorp location for sample collection.




Access Your Results Online
3. Access Your Results Online

View your easy-to-read results online in your OnDemand or Labcorp PatientTM accounts, including Linked Accounts (click here for more details). For certain results that require prompt attention, you will be also be contacted by PWN health via phone or mail.